Clinical and epithelial physiological studies in both cases were normal, suggesting that the substitution of cysteine for phenylalanine at position 508, the F508C mutation, is benign. In their paper, two F508/F508C compound heterozygous individuals were reported. (1990) have described missense variations in the conserved region of exon 10 of the cystic fibrosis (CF) transmembrane conductance regulator gene. Missense variations in the cystic fibrosis gene: Heteroduplex formation in the F508C mutationĭOE Office of Scientific and Technical Information (OSTI.GOV) We infer that coacquisition of multiple strains is common in patients with IM, although only 1 strain tends to be rescued in vitro whether nonrescued strains are present in low abundance or are transformation defective remains to be determined. If the diversity between gp41 sequences was low (20 isolates/patient. A 400-base pair fragment within the envelope gp41 region of the HIV proviral genome was PCR amplified and HMA was applied to both partners' amplicons separately (autologous) and as a mixture (heterologous). A total of 102 HIV-1-infected Zambian and Rwandan couples, with known linkage, were analyzed by gp41-HMA. Here we describe a rapid, cost-effective approach to determine transmission linkage based on the heteroduplex mobility assay (HMA), and validate this approach by comparison to nucleotide sequencing.
To date, this has been accomplished by comparison of polymerase chain reaction (PCR)-amplified nucleotide sequences from potential transmission pairs, which can be cost-prohibitive for use in resource-limited settings.
Manigart, Olivier Boeras, Debrah I Karita, Etienne Hawkins, Paulina A Vwalika, Cheswa Makombe, Nathan Mulenga, Joseph Derdeyn, Cynthia A Allen, Susan Hunter, EricĪ critical step in HIV-1 transmission studies is the rapid and accurate identification of epidemiologically linked transmission pairs. A gp41-based heteroduplex mobility assay provides rapid and accurate assessment of intrasubtype epidemiological linkage in HIV type 1 heterosexual transmission Pairs.
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